Figure 1. Intracellular calcium mobilization of 10 μM VIP on transient-transfected HEK293/Gα15 cells with pcDNA3.1-VPAC2 plasmid.
HEK293/Gα15 Stable Cell Line
HEK293/Gα15 is a HEK293 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein. It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium. The cell line carries the puromycin resistance gene and is resistant to puromycin
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