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目录产品 » 稳定细胞系 » Human Recombinant CD32A 131His Stable Cell Line

Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

CHO-K1/human CD32A 131His Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131His). The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study.

产品编号	M00598
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from NM_001136219.1; no expressed tags
Target Protein	NP_001129691.1
Host Cell	CHO-K1
Quantity	Two vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms	CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Background	CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Monoclonal antibodies can distinguish between CD32A and CD32B.