<dd id='IyFvW'></dd>

<bdo id='n6rlb'><sup id='n6rlb'><div id='n6rlb'><bdo id='n6rlb'></bdo></div></sup></bdo>

目录产品 » 稳定细胞系 » Human Recombinant CD32B 232Thr Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD32B 232 Thr on CHO-K1/CD32B 232 Thr cells.
The CHO-K1/CD32B 232 Thr cells (blue) and the negative control CHO-K1 cells (red) were stained with APC anti-human CD32 antibody (Cat. No. 303208, BioLegend).

CHO-K1/CD32B 232Thr Stable Cell Line

Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Thr). The surface expression of CD32B 232Thr is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD32B 232Ile. GenScript also offers CD32B 232Ile overexpression stable cell line (Cat. No. M00587) for FcγRIIb polymorphism study.

产品编号	M00600
规格	2 vials 大单询价
数量
价格	询价


联系黄金城集团

产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from KR710627.1; no expressed tags
Target Protein	AKI71009.1
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)

技术背景

Gene Synonyms	CD32; FCG2; CD32B; FCGR2; IGFR2
Background	FCGR2B (CD32B) is a low affinity receptor for Immunoglobulin G (IgG). Mutation in the gene in humans leads to a lupus phenotype. The cytoplasmic part of this receptor contains an immunoreceptor tyrosine-based inhibitory motif, in contrast to the activating isoform, FCGR2A (CD32A).