![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_FACS Analysis.png&file=scm_files/productFile_notes/2022/04/08/44bb5e73-e2b0-49ec-b22c-52fe117bac00.png)
Figure 1: FACS analysis of cell surface expression of ACE2 on HEK293 cells.
HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Binding Assay.png&file=scm_files/productFile_notes/2022/04/08/f7d601a8-dc55-4252-8e6f-51dd165a1054.png)
Figure 2: Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells.
HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], mAb, Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.071 ug/ml.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Neutralization Assay.png&file=scm_files/productFile_notes/2022/04/08/729441d3-f98f-4673-8c83-3d8859c76802.png)
Figure 3: Neutralization assay of SARS-CoV-2 spike protein RBD by ACE2-Fc fusion proteins on HEK293/ACE2 cells.
HEK293/ACE2 cells were incubated with 1 µg/ml SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript), and ACE2-Fc fusion protein (Cat. No. Z03484, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], mAb, Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Stability Validation.png&file=scm_files/productFile_notes/2022/04/08/15b5ed0b-d988-4c42-b507-01c5ba1ad380.png)
Figure 5: FACS analysis of cell surface expression of ACE2 on HEK293 cells from different passages.
The HEK293/ACE2 cells and the negative control HEK293 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 represent cell passage numbers.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Pseudovirus Transfection Assay.png&file=scm_files/productFile_notes/2022/04/14/7ffabdea-e1ff-4034-87c3-35fb6fbf7905.png)
Figure 4: SARS-CoV-2 (B.1.1.529, Omicron) pseudovirus transfection on HEK293/ACE2 cells.
SARS-CoV-2 pseudovirus with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between RBD and ACE2 on HEK293 cells
HEK293/ACE2 Stable Cell Line
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