| Product Description |
CytoSinct CD3 Nanobeads, human is used for in vitro enrichment or depletion of CD3+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspen-sion based on the surface expression of human CD3.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet.
The CD3+ cells labeled with Nanobeads are retained within the column and unlabelled cells (CD3- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD3+ cells can be eluted as the positively selected cell fraction.
| Cat. No. | Product Name | Size | Capacity |
|---|
| L00896-0.5 | CytoSinct™ CD3 Nanobeads, human | 500 μL | Up to 5×108 total cells
| | L00896-1 | CytoSinct™ CD3 Nanobeads, human | 1 mL | Up to 1×109 total cells
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| Format |
Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188. |
| Components |
1 mL or 0.5 mL CytoSinct CD3 Nanobeads, human Nanobeads conjugated to monoclonal anti-human CD3 antibodies (isotype: mouse IgG2a). |
| Storage |
Store at 2-8 °C. Do not freeze.
|
T cells were enriched or depleted from human PBMCs. CD3+ cells were stained with anti-CD3 (clone:UCHT1) PE and gated on Live/hCD45+.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
