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CBEmax mRNA (Cap1, m1Ψ)

This mRNA is human codon optimized CBE4max SpCas9 variant named SpG, with sequence originally from Walton et al Science. 2020 Apr 17;368(6488):290-296, capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudouridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
RP-A00002
￥1000

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产品简介
产品介绍 Cytosine base editors (CBEs) system is constructed by fusing an artificially evolved cytosine deaminase to a mutated Cas9 that is a single-strand DNA nickase, can precisely and permanently convert C·G to T·A with the guidance of a target-specific guide RNA (gRNA) without creating a double-strand DNA break and without requiring an exogenous DNA repair donor. This mRNA is human codon optimized CBE4max SpCas9 variant named SpG, with sequence originally from Walton et al Science. 2020 Apr 17;368(6488):290-296, capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1- methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA. This mRNA is human codon optimized CBE4max SpCas9 variant named SpG, with sequence originally from Walton et al Science. 2020 Apr 17;368(6488):290-296, capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
产品浓度 1mg/mL
存储溶液 1mM Sodium citrate, pH6.5
mRNA序列全长 5831nt
mRNA全长分子量 1899231
储存条件 Store at -20°C for short term (<3 months), store at -80°C for long term.

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