The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of LP01. The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudoUridine. It expresses an enhanced green fluorescent protein (Ex: 488nm, Em: 507nm). This formulation is a good control for testing if LP01 LNP is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.