PiggyBac is a DNA transposon and a promising alternative to Sleeping Beauty. Piggybac is originally isolated from the cabbage looper moth Trichoplusia ni genome, is distinguished by its ability to excise precisely. ( Zheng Zhang, et al, J Comput Biol 2000; 7(1-2):203-14.) This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudo Uridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.