| Species |
Mouse |
| Protein Construction |
GH (Phe27-Phe216)
Accession # P06880 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by SEC-HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Recombinant mouse growth hormone is fully biologically active when compared to standard human growth hormone which is 3.0 units/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
22 kDa |
| N-terminal Sequence Analysis |
The sequence of the five N-terminal amino acids was determined and found to be Met-Phe-Pro-Ala-Met. |
| Formulation |
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 500 mM NaCl buffer. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 µg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Growth Hormone (GH), is a member of the somatotropin / prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. |
| Synonyms |
GH1; GH; GHN; GH-N; hGH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
