| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met.
FGF-18 (Ala27-Arg199)
Accession # O76093 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 10.0 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 1.0 × 105 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~20.3 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Fibroblast Growth Factor-18 (FGF-18) is a heparin-binding growth factor that is a member of the FGF family. FGF-18 signals through FGFR 1c, 2c, 3c, and 4. FGF-18 plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. FGF-18 is required for normal ossification and bone development. It can also stimulate hepatic and intestinal proliferation. |
| Synonyms |
FGF18; Fibroblast Growth Factor-18; zFGF5; FGFI |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
