| Species |
Rat |
| Protein Construction |
Expressed with an N-terminal Met.
IL-18 (His37-Ser194)
Accession # P97636-1 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 2.0 μg/ml resulted in the secretion of 0.15 ng/ml h-IFN-gamma when tested using KG-1 cell line, corresponding to a specific activity of > 500.0 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~18.4 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against 50 mM Tris, 50 mM NaCl, pH 8.0. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. |
| Synonyms |
IL18; IGIF; IL-18; IL-1g; IL1F4; interleukin 18 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
