Western blot analysis of SARS-CoV-2 Spike protein (S1) (GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501

Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).

Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE

SARS-CoV-2 Spike protein (S1)

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity.

产品编号	Z03501
规格	100 μg 1 mg 大单询价
数量
价格	￥3800


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产品简介

Species

SARS-CoV-2

Protein Construction

S1 protein (Gln14-Arg685)
Accession # P0DTC2

Purity

> 90% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

SARS-CoV-2 Spike protein (S1) can bind with Human ACE2 in functional ELISA assay.

Expression System

293 Cells

Theoretical Molecular Weight

78.3 kDa
(Additional amino acids from the vector are included. The sequence of them is confidential.)

Formulation

Supplied as a solution in PBS pH 7.4

Concentration

Please refer to the COA for the specific lot.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.

图例

Western blot analysis of SARS-CoV-2 Spike protein (S1) (GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501

Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).

Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE

技术背景

Target Background

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity.

Synonyms

SARS-CoV-2 S1 protein; 2019-nCoV S1 protein

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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SARS-CoV-2 Spike protein (S1)

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