| Species |
Human |
| Protein Construction |
Siglec-2/CD22 (Asp20-Arg687)
Accession # P20273 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
≤ 1 EU/μg of protein by LAL method |
| Biological Activity |
Immobilized Human Siglec2 at 0.5 μg/ml (100 μl/Well). Dose response curve for Biotinylated Anti-Siglec2 Ab with the EC50 of 0.2 μg/ml determined by ELISA. |
| Expression System |
Expi293 |
| Apparent Molecular Weight |
120~130 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Avoid repeated freeze-thaw cycles. |
Immobilized Human Siglec2 at 0.5 μg/ml (100 μl/Well). Dose response curve for Biotinylated Anti-Siglec2 Ab with the EC50 of 0.2 μg/ml determined by ELISA.
Recombinant Siglec-2/CD22 on SDS-PAGE under reducing conditions. The purity is greater than 95%.
The purity of Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC
| Target Background |
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
| Synonyms |
CD22 molecule; CD22; Siglec2; Siglec-2;B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
