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A new cell line for high throughput HIV-specific antibody-dependent cellular cytotoxicity (ADCC) and cell-to-cell virus transmission studies.

J Immunol Methods. . 2016-06;  433:51-8
Orlandi C, Flinko R, Lewis GK. The Institute of Human Virology of the University of Maryland School of Medicine, Baltimore, MD, USA.
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摘要

Several lines of evidence indicate that antibody-dependent cellular cytotoxicity (Wren et al., 2013) is important in the pathogenesis of HIV-1 infection. Namely, ADCC is induced during natural HIV-1 infection or in HIV-1 vaccine studies, the latter demonstrated by the RV144 vaccine trial. To expedite the assessment of ADCC in studies of HIV, we have developed a high throughput assay. We have optimized the rapid fluorometric antibody-mediated cytotoxicity assay (RFADCC) by transfecting the EGFP-CEM-NKr cell line to constitutively express SNAP-tagged CCR5. This cell line can then serve as a source of HIV-specific targets when coated with monomeric gp120, spinoculated with inactivated intact virions, infected by c... More

关键词

EGFP-CEM-NKr-CCR5-SNAP; Flow-cytometry; HIV-1 virus; Monoclonal antibody; RFADCC