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Nerve growth factor promotes expression of costimulatory molecules and release of cytokines in dendritic cells involved in Th2 response through LPS-induced p75NTR

J Asthma.. 2016-12; 
H Tan, P Pan, L Zhang, Z Cao, B Liu, H Li, X Su.
Products/Services Used Details Operation
... purchased from Sigma. Detoxi-Gel Endotoxin Removing Gel (20344) was purchased from Invitrogen. A ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (L00350) was purchased from GenScript. Anti-mouse CD11c fluorescein ...

摘要

INTRODUCTION: Nerve growth factor (NGF) plays an important role in asthmatic inflammatory responses. However, the effects of NGF on dendritic cells (DCs) in asthmatic inflammation remain unknown. Therefore, we examined the effects of NGF on co-stimulatory molecules and the release of cytokines after ovalbumin (OVA) and a low dose of LPS (low LPS) stimulation of dendritic cells. METHODS: Bone-marrow-derived dendritic cells (BMDCs) were collected from 6- to 8-week-old wide or TLR4(-/-) mice. BMDCs were treated with OVA and/or low LPS for 12h, and then stimulated with NGF for 24h. ELISA and flow cytometry were performed to measure TSLP, IL-6, IL-10, and IL-12 production and MHCII and CD86 expression on BMDCs. BM... More

关键词

Nerve growth factor; Th2 response; dendritic cells; ovalbumin