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Generation and characterization of human B lymphocyte stimulator blocking monoclonal antibody.

Mol Immunol.. 2016-09; 
Zhuang W, Zhang J, Pei L, Fang S, Liu H, Wang R, Su Y.
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... For additional functional assays, monoclonal antibodies were purified from clonal supernatant by protein G affinity chromatography (Genscript, L00209). 2.2. Humanization and maturation of anti-Blys mAbs. The cDNAs from the hybridomas were amplified by RT-PCR. ...

摘要

The cytokine, B lymphocyte stimulator (Blys) is essential for activation and proliferation of B cells and is involved in the pathogenesis of B-cell mediated autoimmune diseases. Based on its essential activity, Blys may be a potential therapeutic target for human autoimmune diseases. In this article, we have described the development of a novel humanized anti-Blys antibody, NMB04, that binds with high affinity and specificity to both soluble and membrane bound Blys. This monoclonal antibody has the potential to block Blys binding to all its three receptors, TACI, BCMA and BR-3. Further in vivo studies revealed that NMB04 possessed more potent inhibitory activity against human Blys as compared to an existing ant... More

关键词

Antibody; Autoimmune; Blys; SLE