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Efficient increase of the novel recombinant human plasminogen activator expression level and stability through the use of homozygote transgenic rabbits.

Int. J. Mol. Med.. 2018-10; 
HeZhengyi,JiangLei,ZhangTing,ZhouMinya,WuDaijin,YuanTingting,YuanYuguo,Cheng
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… no. A01854-200; 1:1,000; GenScript, Piscataway, NJ, USA) for 2 h at 37˚C. Finally, the membrane was washed three times with the wash buffer, and the reaction was developed with dAB colorimetric solution reagent. Results Generation of homozygous rhPA transgenic rabbits …

摘要

Expression efficacy of recombinant protein in current expression systems is generally low. Therefore, the expression levels of recombinant proteins in the breast milk of transgenic animals are typically low. In view of this, the present study aimed to construct homozygous transgenic rabbits with a high expression level of recombinant human plasminogen activator (rhPA) during the entire lactation period. Homozygous transgenic rabbits were obtained using an effective rhPA mammary‑specific expression vector PCL25/rhPA. The expression level and thrombolytic ability of rhPA in the milk of both homozygous and hemizygous rabbits were detected by enzyme‑linked immunosorbent and fibrin agarose plate assays. It w... More

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