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Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration.

Int. J. Biol. Sci.. 2018; 
ZhangYing,YiFei,WangLiang,WangZhuo,ZhangNaijin,WangZhijun,LiZiwei,SongXiaoyu,WeiShi,Ca
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… h. Then the membranes were incubated with rabbit anti-SMC1 (ab21583, Abcam), rabbit anti-SMC1 (phospho S966) (ab1276, Abcam), rabbit anti-PCNA (10205-2-AP, Proteintech), rabbit anti-MMP9 (10375- 2-AP, Proteintech) or mouse anti-flag (A00187-100, Genscript) at 4 °C …

摘要

Structural maintenance of chromosomes protein 1A (SMC1A) has been implicated in the development of a variety of cancer types. However, its role in hepatocellular carcinoma remains unknown. In this study, we found that phosphorylated SMC1A was highly expressed in HepG2 and Bel7402 cells when compared with other cancer cell lines. Furthermore, SMC1A knockdown dramatically reduced HepG2 and Bel7402 cell proliferation and migration. Re-expressing phosphomimetic mutants S957DS966D significantly enhanced the proliferation and migration of SMC1A knockdown HepG2 and Bel7402 cells. In addition, phosphorylated SMC1A promotes hepatocellular carcinoma cells growth . Importantly, the expression of phosphorylated S... More

关键词

Hepatocellular Carcinoma,Migration,Prognosis,Proliferation,SMC1 phosphoryla