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Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound.

Metab. Eng.. 2018-09; 
Englund Elias,Shabestary Kiyan,Hudson Elton P,Lindber
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Catalog Antibody … After separation and blotting, detection of the proteins were done using a different anti-Strep-tag antibody than for E. coli (Abcam, ab76949), an anti-His-tag antibody (Genscript, A00186) or an anti-FLAG-tag antibody (Sigma-Aldrich, F3165) … Get A Quote

摘要

Of the two natural metabolic pathways for making terpenoids, biotechnological utilization of the mevalonate (MVA) pathway has enabled commercial production of valuable compounds, while the more recently discovered but stoichiometrically more efficient methylerythritol phosphate (MEP) pathway is underdeveloped. We conducted a study on the overexpression of each enzyme in the MEP pathway in the unicellular cyanobacterium Synechocystis sp. PCC 6803, to identify potential targets for increasing flux towards terpenoid production, using isoprene as a reporter molecule. Results showed that the enzymes Ipi, Dxs and IspD had the biggest impact on isoprene production. By combining and creating operons out of th... More

关键词

Carbon flux,Cyanobacteria,Isoprene,MEP pathway,Metabolic engineering,Metabolic mode