黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Binge alcohol alters PNPLA3 levels in liver through epigenetic mechanism involving histone H3 acetylation

Alcohol. 2017-05; 
RestrepoRicardo J, LimRobert W, KorthuisRonald J, ShuklaShivend
Products/Services Used Details Operation
… C. The protocol used for hepatocyte ChIP was adapted from earlier reports (Browne et al., 2014, Tomita et al., 2003). Magnetic beads from GenScript (Protein A/G MagBeads, GenScript catalog #L00277) were used. In addition …

摘要

The human PNPLA3 (patatin-like phospholipase domain-containing 3) gene codes for a protein which is highly expressed in adipose tissue and liver, and is implicated in lipid homeostasis. While PNPLA3 protein contains regions homologous to functional lipolytic proteins, the regulation of its tissue expression is reflective of lipogenic genes. A naturally occurring genetic variant of PNPLA3 in humans has been linked to increased susceptibility to alcoholic liver disease. We have examined the modulatory effect of alcohol on PNPLA3 protein and mRNA expression as well as the association of its gene promoter with acetylated histone H3K9 by chromatin immunoprecipitation (ChIP) assay in rat hepatocytes in vitro, ... More

关键词

Binge ethanol,Chronic-binge ethanol,Epigenetics,Histone acetylation,PN