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The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack.

Ann. Bot.. 2018; 
KrckováZuzana,KocourkováDaniela,DanekMichal,BrouzdováJitka,PejcharPremysl,JandaMartin,PokotyloIgor,OttPeter G,ValentováOlga,Martine
Products/Services Used Details Operation
Bacterial suspensions (106, 107, 108 or 109 cfu mL−1), 1 μm flg22 (22-amino acid peptide from N-terminal part of flagellin, GenScript, Piscataway, NJ, USA, cat. no. RP19986), 500 μm SA, 50 μm methyljasmonic acid (MeJA) and 5 mm 1-aminocyclopropane-1-carboxylic acid (ACC), or water were infiltrated to the leaves.

摘要

The non-specific phospholipase C (NPC) is a new member of the plant phospholipase family that reacts to abiotic environmental stresses, such as phosphate deficiency, high salinity, heat and aluminium toxicity, and is involved in root development, silicon distribution and brassinolide signalling. Six NPC genes (NPC1-NPC6) are found in the Arabidopsis genome. The NPC2 isoform has not been experimentally characterized so far.

关键词

Arabidopsis thaliana,MAMP-triggered immunity,Pseudomonas syringae,effector-triggered immunity,flagellin,non-specific phospholipase C,phosphatidylcholine-specific phospholipase C,reactive oxygen spe