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Nucleoporin Nup50 Stabilizes Closed Conformation Of Armadillo Repeat 10 In Importin 5.

J Biol Chem.. 2012-01;  287(3):2022-31
Pumroy RA, Nardozzi JD, Hart DJ, Root MJ, Cingolani G. Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
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摘要

The human genome encodes six isoforms of importin α that show greater than 60% sequence similarity and remarkable substrate specificity. The isoform importin α5 can bind phosphorylated cargos such as STAT1 and Epstein-Barr Virus Nuclear Antigen 1, as well as the influenza virus polymerase subunit PB2. In this work, we have studied the interaction of the nucleoporin Nup50 with importin α5. We show that the first 47 residues of Nup50 bind to the C terminus of importin α5 like a "clip," stabilizing the closed conformation of ARM 10. In vitro, Nup50 binds with high affinity either to empty importin α5 or to a preassembled complex of importin α5 bound to the C-terminal d... More

关键词

Influenza Virus; Nuclear PoreNuclear Transport; Signaling; X-ray Crystallography; Armadillo Repeat; PB2; Importin Alpha5; Nup5