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Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.

Sci Rep. 2015; 
HansenHenning Gram,NilssonClaes Nymand,LundAnne Mathilde,KolStefan,GravLise Marie,LundqvistMagnus,RockbergJohan,LeeGyun Min,AndersenMikael Rørdam,KildegaardHelene Faus
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Molecular Biology Reagents … The titer of hEPO in supernatant samples was determined using a hEPO ELISA Kit (#ab119522, Abcam) according to the manufacturer's instructions Purified recombinant hEPO (#Z02975-50, GenScript, Piscataway, NJ, USA) was used as standard … Get A Quote

摘要

Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non-mAb therapeutic glycoproteins is more likely to reduce production costs significantly. The aim of this study was to establish a versatile target gene screening platform for improving productivity for primarily non-mAb glycoproteins with complete interchangeability of model proteins and target genes using transient expression. The platform consists of four techniques compatible with 96-well microplates: lipid-based transient transfection, cell cultivation in m... More

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