黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells.

Mol. Cells. 2016; 
ShresthaPravesh,YunJi-Hye,KimWoo Taek,KimTae-Yoon,LeeWeo
Products/Services Used Details Operation
Biochemicals … G1 Affinity resin (Cat. No. L00432). FLAG-tagged hEC-SOD was then purified following the protocol provided by GenScript. Purified hEC-SOD was simultaneously concentrated to 0.3 mM (9 mg/ml). The final buffer was exchanged Get A Quote

摘要

A balance between production and degradation of reactive oxygen species (ROS) is critical for maintaining cellular homeostasis. Increased levels of ROS during oxidative stress are associated with disease conditions. Antioxidant enzymes, such as extracellular superoxide dismutase (EC-SOD), in the extracellular matrix (ECM) neutralize the toxicity of superoxide. Recent studies have emphasized the importance of EC-SOD in protecting the brain, lungs, and other tissues from oxidative stress. Therefore, EC-SOD would be an excellent therapeutic drug for treatment of diseases caused by oxidative stress. We cloned both the full length (residues 1-240) and truncated (residues 19-240) forms of human EC-SOD (hEC-... More

关键词

LC-MS/MS,extracellular matrix (ECM),human extracellular superoxide (hEC-SOD),reactive oxygen species (ROS),sodiumdiethyldithiocarba