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Porcine Mx1 Protein Inhibits Classical Swine Fever Virus Replication by Targeting Nonstructural Protein NS5B.

J. Virol.. 2018; 
ZhouJing,ChenJing,ZhangXiao-Min,GaoZhi-Can,LiuChun-Chun,ZhangYun-Na,HouJin-Xiu,LiZhao-Yao,KanLin,LiWen-Liang,Zho
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Catalog Antibody The bound proteins were analyzed by Western blotting using mouse anti-GST MAb (A00865-100; Genscript) and mouse anti-Flag MAb (F1804; Sigma-Aldrich). Get A Quote

摘要

Mx proteins are interferon (IFN)-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses; they belong to the dynamin superfamily of large GTPases. In this study, we confirmed the anti-classical swine fever virus (CSFV) activity of porcine Mx1 and showed that porcine Mx2 (poMx2), human MxA (huMxA), and mouse Mx1 (mmMx1) also have anti-CSFV activity Small interfering RNA (siRNA) experiments revealed that depletion of endogenous poMx1 or poMx2 enhanced CSFV replication, suggesting that porcine Mx proteins are responsible for the antiviral activity of interferon alpha (IFN-α) against CSFV infection. Confocal microscopy, immunoprecipitation, glutathione -transfer... More

关键词

antivirus,classical swine fever virus (CSFV),mutants,nonstructural protein 5B (NS5B),porcine Mx1 (po