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Structural and functional analysis of the GABARAP interaction motif (GIM).

EMBO Rep.. 2017; 
Rogov Vladimir V,Stolz Alexandra,Ravichandran Arvind C,Rios-Szwed Diana O,Suzuki Hironori,Kniss Andreas,L?hr Frank,Wakatsuki Soichi,D?tsch Volker,Dikic Ivan,Dobson Renwick Cj,McEwan Dav
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摘要

Through the canonical LC3 interaction motif (LIR), [W/F/Y]-X-X-[I/L/V], protein complexes are recruited to autophagosomes to perform their functions as either autophagy adaptors or receptors. How these adaptors/receptors selectively interact with either LC3 or GABARAP families remains unclear. Herein, we determine the range of selectivity of 30 known core LIR motifs towards individual LC3s and GABARAPs. From these, we define a ABARAP nteraction otif (GIM) sequence ([W/F]-[V/I]-X-V) that the adaptor protein PLEKHM1 tightly conforms to. Using biophysical and structural approaches, we show that the PLEKHM1-LIR is indeed 11-fold more specific for GABARAP than LC3B. Selective mutation of the X and X pos... More

关键词

Atg8,GABARAP,LC3,PLEKHM1,autop