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Src kinase phosphorylates Notch1 to inhibit MAML binding.

Sci Rep. 2018; 
LaFoya Bryce,Munroe Jordan A,Pu Xinzhu,Albig All
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Catalog Antibody Primary antibodies against FLAG (DYKDDDDK) tag (A00170) were purchased from GenScript.... For immunoprecipitation of FLAG tagged proteins, the remaining 450 µL of lysate was incubated with 20 µL of anti-FLAG G1 affinity resin (GenScript) overnight on a tube rotator at 4 °C. Get A Quote

摘要

Notch signaling is a form of intercellular communication which plays pivotal roles at various stages in development and disease. Previous findings have hinted that integrins and extracellular matrix may regulate Notch signaling, although a mechanistic basis for this interaction had not been identified. Here, we reveal that the regulation of Notch by integrins and extracellular matrix is carried out by Src family kinases (SFKs) working downstream of integrins. We identify a physical interaction between the SFK member, c-Src, and the Notch intracellular domain (NICD) that is enhanced by β3 integrin and the integrin binding ECM protein, MAGP2. Our results demonstrate that c-Src directly phosphorylates t... More

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