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Interplay between RNA-binding protein HuR and microRNA-125b regulates p53 mRNA translation in response to genotoxic stress.

RNA Biol. 2016; 
Ahuja Deepika,Goyal Ashish,Ray Partho Sar
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Catalog Antibody 45 Cell lysates were quantified using Bradford reagent (Amresco , E530-1L), resolved on 12% SDS-PAGE and were immunoblotted using ant i HuR (3A2, Santa Cruz Biotechnology), p53 (DO-1,Santa Cruz Biotechno logy), Ago2 (C34C6, Cell Signalling), Lamin A/C (4C11, Cell Signaling), -actin (A00730, Genscript) and GAPDH (FL- 335, Santa Cruz Biotechno logy) ant ibodies. Get A Quote

摘要

Tumor suppressor protein p53 plays a crucial role in maintaining genomic integrity in response to DNA damage. Regulation of translation of p53 mRNA is a major mode of regulation of p53 expression under genotoxic stress. The AU/U-rich element-binding protein HuR has been shown to bind to p53 mRNA 3'UTR and enhance translation in response to DNA-damaging UVC radiation. On the other hand, the microRNA miR-125b is reported to repress p53 expression and stress-induced apoptosis. Here, we show that UVC radiation causes an increase in miR-125b level in a biphasic manner, as well as nuclear cytoplasmic translocation of HuR. Binding of HuR to the p53 mRNA 3'UTR, especially at a site adjacent to the miR-125b targ... More

关键词

Cancer,DNA damage,HuR,RNA-binding protein,miR-125b,microRNA,p53,translation regula