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DNA sense-and-respond protein modules for mammalian cells.

Nat. Methods. 2015; 
Slomovic Shimyn,Collins Jam
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Proteins, Expression, Isolation and Analysis Membranes were probed with either α-Flag-M2 (Sigma-Aldrich, F3165) or α-HIS (GenScript, A00174-40) primary antibodies and secondary HRP-conjugated antibodies (Bio-Rad, #1721011 and #1706515), and imaged with a Gel Logic system (Carestream Mol. Get A Quote

摘要

We generated synthetic protein components that can detect specific DNA sequences and subsequently trigger a desired intracellular response. These modular sensors exploit the programmability of zinc-finger DNA recognition to drive the intein-mediated splicing of an artificial trans-activator that signals to a genetic circuit containing a given reporter or response gene. We used the sensors to mediate sequence recognition-induced apoptosis as well as to detect and report a viral infection. This work establishes a synthetic biology framework for endowing mammalian cells with sentinel capabilities, which provides a programmable means to cull infected cells. It may also be used to identify positively transduced or... More

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