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The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry

J Virol. 2019-11; 
Guangwen Wang, Li Jiang, Jinliang Wang , Jie Zhang, Fandi Kong, Qibing Li , Ya Yan, Shanyu Huang, Yuhui Zhao, Libin Liang , Junping Li , Nan Sun, Yuzhen Hu, Wenjun Shi , Guohua Deng , Pucheng Chen, Liling Liu, Xianying Zeng, Guobin Tian, Zhigao Bu, Hualan Chen, Chengjun Li
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Catalog Antibody mouse anti-GST mAb (A00865, Genscript, Nanjing, China)... rabbit anti-GST pAb (A00097, Genscript), Get A Quote

摘要

Influenza A virus (IAV) co-opts numerous host factors to complete its replication cycle. Here, we identified free fatty acid receptor 2 (FFAR2) as a cofactor for IAV entry into host cells. We found that downregulation of FFAR2 or Ffar2 expression significantly reduced the replication of IAV in A549 or RAW 264.7 cells. The treatment of A549 cells with FFAR2 siRNA or the FFAR2 pathway agonists 4-CMTB and compound 58 [Cmp 58; (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide], dramatically inhibited the nuclear accumulation of viral NP protein at early timepoints post infection, indicating that FFAR2 functions in the early stage of IAV replication cycle. FFAR2 downregulation had no effect on t... More

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