Filoviruses， which include ebolaviruses and marburgvirus， can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates， with human fatality rates reaching 90% during some outbreaks. Currently， there is lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases， there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known and species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs， which demonstrates the potential for use in diagnostics.