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Molecular optimization of autotransporter-based tyrosinase surface display.

Biochim Biophys Acta Biomembr. 2019; 
H?rnstr?mDavid,LarssonGen,van MarisAntonius J A,GustavssonMa
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摘要

Display of recombinant enzymes on the cell surface of Gram-negative bacteria is a desirable feature with applications in whole-cell biocatalysis, affinity screening and degradation of environmental pollutants. One common technique for recombinant protein display on the Escherichia coli surface is autotransport. Successful autotransport of an enzyme largely depends on the following: (1) the size, sequence and structure of the displayed protein, (2) the cultivation conditions, and (3) the choice of the autotransporter expression system. Common problems with autotransporter-mediated surface display include low expression levels and truncated fusion proteins, which both limit the cell-specific activity. T... More

关键词

Autotransport,Escherichia coli,Protein engineering,Tyrosinase,Whole-cell biocatal