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A novel pathway of LPS uptake through syndecan-1 leading to pyroptotic cell death.

Elife. 2018; 
YokoyamaShigetoshi,CaiYan,MurataMiyuki,TomitaTakeshi,YonedaMitsuhiro,XuLei,PilonAprile L,CachauRaul E,KimuraSh
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Cellular Analysis LPS quantification in each SCGB3A2 recombinant protein was performed using the ToxinSensorTMChromogenic LAL Endotoxin Assay Kit (L00350, GenScript). Get A Quote

摘要

Intracellular lipopolysaccharide (LPS) triggers the non-canonical inflammasome pathway, resulting in pyroptosis of innate immune cells. In addition to its well-known proinflammatory role, LPS can directly cause regression of some tumors, although the underlying mechanism has remained unknown. Here we show that secretoglobin(SCGB)3A2, a small protein predominantly secreted in airways, chaperones LPS to the cytosol through the cell surface receptor syndecan-1; this leads to pyroptotic cell death driven by caspase-11. SCGB3A2 and LPS co-treatment significantly induced pyroptosis of macrophage RAW264.7 cells and decreased cancer cell proliferation in vitro, while SCGB3A2 treatment resulted in reduced pr... More

关键词

LPS,cancer cell death,immunology,inflammation,mouse,non-canonical infammasome pathway,pyroptosis,secretoglobin3A2,syndec