| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … lysed on ice, aliquots of cleared cell lysates were collected as input controls, and the remaining lysates were incubated with NF-YA and MDR1b antibodies overnight at 4 °C The lysates were then mixed with prewashed Protein A/G Sepharose beads (GenScript, L00277) for 3 h … | Get A Quote |
Cadmium (Cd) has been reported to induce reproductive toxicity. Recent study indicated that aberrant epigenetic regulation of Multidrug resistance 1b (Mdr1b) causes xenobiotic efflux failure at the blood-testis barrier (BTB). However, whether Mdr1b dysregulation is involved in Cd-mediated dyszoospermia and the underlying mechanism remain unknown. In this study, mice were intragastrically administered 0 or 2.5 mg/kg CdCl2 every other day for 2 months to investigate changes in spermatogenesis and epigenetic regulation of Mdr1b. Mouse Leydig cells TM3 were cultured to detect Mdr1b expression localization. We found that the Cd group revealed BTB disruption concomitant with obvious sperm abnormity and dynamic impai... More
