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Protective effects of Progranulin against focal cerebral ischemia-reperfusion injury in rats by suppressing endoplasmic reticulum stress and NF-κB activation in reactive astrocytes.

J Cell Biochem. 2018; 
Shu Q, Fan H, Li SJ, Zhou D, Ma W, Zhao XY, Yan JQ, Wu G.
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Catalog Antibody Samples were blocked using 5% skim milk at room temperature for 1 h, incubated at a temperature of 4°C overnight with primary antibodies of PGRN (1:1000; ab19121), GFAP (1:1000; ab7260), GRP78 (1:1000; ab21685), CHOP (1:1000; ab11419), NF-κB p65 (1:1000; ab207297) (all from Abcam Inc.) and β-actin (1:2000 A00702; GenScript Inc., New Jersey). Get A Quote

摘要

The aim of this study is to explore the effect progranulin (PGRN) has on endoplasmic reticulum (ER) stress and the NF-κB activation in reactive astrocytes found in rat models with focal cerebral ischemia-reperfusion (I/R) injury. Sprague-Dawley (SD) rats were grouped into the sham, I/R, PGRN-high dose, PGRN-low dose, and negative control (NC) groups. TTC staining was applied in order to detect the cerebral infarction volume, a TUNEL assay to detect the apoptosis rate of neurons, an ELISA to measure MDA, SOD, iNOS, LDH, TNF-α, IL-1β, IL-6, and IL-10 levels, and both RT-qPCR and western blotting methods in order to detect PGRN, GFAP, GRP78, CHOP, and NF-κB p65 expressions. The astrocytes (AST) cells were then... More

关键词

Endoplasmic reticulum (ER) stress; astrocyte; focal cerebral ischemia-reperfusion; nuclear factor kappa B; progranulin (PGRN)