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Atomic insights into the genesis of cellular filaments by globular proteins.

Nat Struct Mol Biol. 2018; 
McPartland L, Heller DM, Eisenberg DS,,,,, Hochschild A, Sawaya MR,,,,.
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Monoclonal Antibody Services Blots prepared in duplicate were probed either with an anti-His mouse monoclonal antibody (Genscript A00612), to detect His6-tagged GFP-fusion protein, or with an antibody specific for the RpoA (α ) subunit of E. Get A Quote

摘要

Self-assembly of proteins into filaments, such as actin and tubulin filaments, underlies essential cellular processes in all three domains of life. The early emergence of filaments in evolutionary history suggests that filament genesis might be a robust process. Here we describe the fortuitous construction of GFP fusion proteins that self-assemble as fluorescent polar filaments in Escherichia coli. Filament formation is achieved by appending as few as 12 residues to GFP. Crystal structures reveal that each protomer donates an appendage to fill a groove between the two following protomers along the filament. This exchange of appendages resembles runaway domain swapping but is distinguished by higher efficiency b... More

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