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Establishment of an inducible HBV stable cell line that expresses cccDNA-dependent epitope-tagged HBeAg for screening of cccDNA modulators.

Antiviral Res. 2016; 
Cai D, Wang X, Yan R, Mao R, Liu Y, Ji C, Cuconati A, Guo H.
Products/Services Used Details Operation
Catalog Antibody Chemiluminescence ELISA (CLIA) detection of HA-HBeAg Polystyrene 96-well plate (Cat# 3904, Corning) was coated with 100 ml of anti-HA antibody (cat#: A01244, Genscript; 5 mg/ml in PBS buffer) at 4  C overnight, followed by 5 washes with 150 ml PBS. Get A Quote

摘要

Hepatitis B virus (HBV) covalently closed circular (ccc) DNA is essential to the virus life cycle, its elimination during chronic infection is considered critical to a durable therapy but has not been achieved by current antivirals. Despite being essential, cccDNA has not been the major target of high throughput screening (HTS), largely because of the limitations of current HBV tissue culture systems, including the impracticality of detecting cccDNA itself. In response to this need, we have previously developed a proof-of-concept HepDE19 cell line in which the production of wildtype e antigen (HBeAg) is dependent upon cccDNA. However, the existing assay system is not ideal for HTS because the HBeAg ELISA cross... More

关键词

HBeAg; Hepatitis B virus; High throughput screening; cccDNA