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Porcine Circovirus Type 2 Rep Enhances IL-10 Production in Macrophages via Activation of p38-MAPK Pathway.

Viruses. 2019; 
Wu X, Wang X, Shi T, Luo L, Qiao D, Wang Z, Han C, Du Q, Tong D, Huang Y.
Products/Services Used Details Operation
Catalog Antibody … 12540; CST), anti-Sp1 (9389; CST), anti-c-jun (9165; CST), anti-VG5Q (abs102222; absin, Shanghai, China), anti-TDG (ab154192; abcam, Cambridge, MA, USA), anti-ZNF265 (abs130552; absin), anti-c-Myc (13987; CST), and anti-β-actin (A00702; Genscript, Nanjing, China) … Get A Quote

摘要

Porcine circovirus type 2 (PCV2) is one of the major threats to pig farms worldwide. Although PCV2 has been identified to promote IL-10 production, the detailed regulatory roles of PCV2 Rep for IL-10 production remain unclear. Herein, we first found that PCV2 Rep, rather than PCV1 Rep, enhanced IL-10 expression at the later phase of PCV2 infection in porcine alveolar macrophages (PAMs). Furthermore, we found that PCV2 Rep directly activated the p38-MAPK pathway to promote transcription factors NF-κB p50 and Sp1 binding to the il10 promoter, but PCV1 Rep did not. During PCV2 infection, however, PCV2 Rep promoted the binding activities of NF-κB p50 and Sp1 with the il10 promoter only at the later phase of PCV2 ... More

关键词

Rep; interleukin-10 (IL-10); p38-MAPK pathway; porcine circovirus type 2 (PCV2); thymine DNA glycosylase (TDG)