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Maturation of an Antimicrobial Peptide Inhibits Aeromonas hydrophila Infection in Crayfish.

J Immunol. 2020; 
Zhao BR, Zheng Y, Gao J, Wang XW,.
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Gene Synthesis … First, the anti- PcnAMP Ab was purified. Generally, the antiserum was diluted with a buffer (0.15 M NaCl, 20 mM Na2HPO4 [pH 8]). The dilution was filtered through a 0.45-mm filter and applied to a 2-ml protein A resin column (L00210; GenScript, Nanjing, China) … Get A Quote

摘要

Rapid synthesis and release of active antimicrobial peptides (AMPs) is an important strategy in innate immune. Processing of the precursor into the active form is a common posttranslational modification of AMPs in mammals. However, in invertebrates, the mechanism of AMP maturation is largely unknown. In the current study, to our knowledge, a novel potential AMP, designated as PcnAMP, was identified because of its significant induction by bacterial infection in the red swamp crayfish (Procambarus clarkii). PcnAMP was cleaved into a short fragment postinfection. Using the purified native peptide, this cleavage was found to be mediated by trypsin after synthesis. Proteolysis produced an N-terminal peptide that exe... More

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