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Mechanism of in vivo activation of the MutLγ-Exo1 complex for meiotic crossover formation

biorxiv. 2019; 
Aurore Sanchez,  Céline Adam,  Felix Rauh,  Yann Duroc,  Lepakshi Ranjha,  Bérangère Lombard,  Xiaojing Mu,  Damarys Loew,  Scott Keeney,  Petr Cejka,  Raphaël Guérois,  Franz Klein,  Jean-Baptiste Charbonnier,  Valérie Borde
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Catalog Antibody The copyright holder for this preprint (which was not peer-reviewed) is the 28 were detected by western blot with anti-His antibody (Genscript, A00186) and anti-Flag antibody (Sigma, F3165) Get A Quote

摘要

Crossovers generated during the repair of programmed double-strand breaks (DSBs) are essential for fertility to allow accurate homolog segregation during the first meiotic division. Most crossovers arise through the asymmetric cleavage of double-Holliday junction (dHJ) intermediates by the MutLγ endonuclease (Mlh1-Mlh3) and an elusive non-catalytic function of Exo1, and require the Cdc5/PLK1 kinase. Here we show in budding yeast that MutLγ forms a constitutive complex with Exo1, and in meiotic cells transiently contacts the upstream MutSγ (Msh4-Msh5) heterodimer. Once recombination intermediates are committed to the crossover repair pathway, MutLγ-Exo1 associates with sites of DSB hotspots, and Exo1 recruit... More

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