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Methyl-β-cyclodextrin inhibits EV-D68 virus entry by perturbing the accumulation of virus particles and ICAM-5 in lipid rafts

Antiviral Res. 2020; 
Jiang Y, Liu S, Shen S, Guo H, Huang H, Wei W.
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Gene Synthesis … The membranes were probed with primary antibodies (anti-EV-D68 VP1 polyclonal antibody [Genetex, GTX132312] and anti-α-tubulin monoclonal antibody [GenScript, A01410] at 4 °C overnight, followed by incubation with secondary antibodies (alkaline phosphatase … Get A Quote

摘要

Enterovirus D68 (EV-D68) is a member of the Picornavirus family and a causative agent of respiratory diseases in children. The incidence of EV-D68 infection has increased worldwide in recent years. Thus far, there are no approved antiviral agents or vaccines for EV-D68. Here, we show that methyl-β-cyclodextrin (MβCD), a common drug that disrupts lipid rafts, specifically inhibits EV-D68 infection without producing significant cytotoxicity at virucidal concentrations. The addition of exogenous cholesterol attenuated the anti-EV-D68 activity of MβCD. MβCD treatment had a weak influence on the attachment of viral particles to the cell membrane but significantly inhibited EV-D68 entry into host cells. We demons... More

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