GenScript, NY) is diluted 1:30,000. The anti-mouse or rabbit IgG HRP conjugated antibody was diluted 1:5000 in TBST containing 5% skin milk and the membrane was washed with TBST for 30 min...">

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Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration.

PLoS Genet.. 2013-06;  9(6):e1003559
J Lee, M Song, S Hong. Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea
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摘要

Rhodopsin has been used as a prototype system to investigate G protein-coupled receptor (GPCR) internalization and endocytic sorting mechanisms. Failure of rhodopsin recycling upon light activation results in various degenerative retinal diseases. Accumulation of internalized rhodopsin in late endosomes and the impairment of its lysosomal degradation are associated with unregulated cell death that occurs in dystrophies. However, the molecular basis of rhodopsin accumulation remains elusive. We found that the novel norpAP24 suppressor, diehard4, is responsible for the inability of endo-lysosomal rhodopsin trafficking and retinal degeneration in Drosophila models of retinal dystrophies. We found that diehard4 enc... More

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