黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Identification of protein-protein interactions using a magnetic modulation biosensing system

Sensors and Actuators B: Chemical. 2020; 
ShiraRothaItzhakZanderbYehuditMichelsonaYossiBen-DavidbEhudBaninbAmosDaniellia
Products/Services Used Details Operation
Catalog Antibody The following antibodies were used in this study: biotinylated anti-hEPOR (MBS2001845, MyBioSource), anti-hEPO (RP1004, Boster Biological Technology Co.), biotinylated anti-human IgG Fc (SSA008, Sino Biological Inc), anti-His tag monoclonal antibody (70796-3, Novagen), anti-FLAG antibody (F3165, Sigma Aldrich), goat anti-human IgG Fc (HRP) (ab97225, Abcam), goat anti-rabbit (HRP) antibody (ab97080, Abcam), goat anti-mouse (HRP) antibody (sc-2005, Santa Cruz), and biotinylated anti-FLAG tag antibody (A01428-100, GenScript). Get A Quote

摘要

Investigating protein-protein interactions (PPIs) is essential in basic and clinical research, but current techniques for detecting PPI are hampered by lengthy protocols, reduced sensitivity, and a high rate of false positive results. To overcome these challenges, we use a magnetic modulation biosensing (MMB) system, in which one protein of interest is attached to a magnetic bead and another protein is coupled to a fluorescent molecule. Thus, when the two proteins interact, the fluorescent molecule is connected to the magnetic bead. An oscillating magnetic field gradient then concentrates the beads and transports them in a periodic motion in and out of a laser beam, producing an oscillating signal that is detec... More

关键词