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Aggregatibacter actinomycetemcomitans LPS binds human interleukin-8

Journal of Oral Microbiology. 2018; 
Tuuli Ahlstrand, Laura Kovesjoki, Terhi Maula, Jan Oscarsson &Riikka Ihalin
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Gene Synthesis The amount of LPS was determined with a ToxiSensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript, L00350), the protein concentration was measured by the method of Lowry and coworkers [22] Get A Quote

摘要

Various gram-negative species sequester host cytokines using outer membrane proteins or surface modulation by sulfated polysaccharides. An outer membrane lipoprotein (BilRI) of the periodontal pathogen Aggregatibacter actinomycetemcomitans binds several cytokines, including interleukin (IL)-8. Because IL-8 is positively charged at physiological pH, we aimed to determine whether IL-8 interacts with negatively charged lipopolysaccharide (LPS). Binding was investigated using electrophoretic mobility shift assays and microwell-based time-resolved fluorometric immunoassay. LPS from each tested strain of A. actinomycetemcomitans (N = 13), Pseudomonas aeruginosa (N = 1) and Escherichia coli (N = 1) bound... More

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