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Application and expression of HSV gG1 protein from a recombinant strain.

J Virol Methods.. 2010-11;  169(2):351-8
Yan H, Yan H, Huang T, Li G, Gong W, Jiao H, Chen H, Ji M. a Department of Microbiology and Immunology, School of Medicine, Yangzhou University, Yangzhou 225001, Chinab Department of Medical Science, College of Resources and Environmental Sciences, Yangzhou 225127, China
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摘要

According to the homologous sequence of glycoprotein G1 (gG1) genes from different strains of herpes simplex virus type 1 (HSV-1), a pair of primers was designed to amplify the gG1 gene fragment by PCR. Both the PCR product and the pGEX-4T-1 vector were digested with EcoR I and Sal I. The gG1 gene fragment was subcloned into the digested pGEX-4T-1 vector to construct a recombinant plasmid (pGEX-4T-1-gG1). The resultant plasmid was identified by dual-enzyme digestion and sequence analysis, and then transformed into Escherichia coli BL21 for expression under the induction of isopropyl β-D-1-thiogalactoside (IPTG). The expressed GST-gG1 fragment was detected by SDS-PAGE and purified by affinity chromatography... More

关键词

Herpes simplex virus; gG1 protein; Expression; Affinity chromatography; ELISA; Vaccines