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The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis

Nucleic Acids Res. 2020-12; 
Liangbing Yuan, Xin Song, Lu Zhang, Yaoguang Yu, Zhenwei Liang, Yawen Lei, Jiuxiao Ruan, Bin Tan, Jun Liu, Chenlong Li
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Catalog Antibody … no. D191048) antibody. Immunoblotting Proteins were resolved on 4–12% protein gels (GenScript, SurePAGE, Cat. no. M00653) and detected by anti- GFP (Abcam, cat. no. ab290, Lot#GR3196305-1), anti- HA (Sigma-Aldrich, cat. no. H6533), anti-FLAG (Sigma- Aldrich, cat … Get A Quote

摘要

The Polycomb repressive complex 2 (PRC2) catalyzes histone H3 Lys27 trimethylation (H3K27me3) to repress gene transcription in multicellular eukaryotes. Despite its importance in gene silencing and cellular differentiation, how PRC2 is recruited to target loci is still not fully understood. Here, we report genome-wide evidence for the recruitment of PRC2 by the transcriptional repressors VIVIPAROUS1/ABI3-LIKE1 (VAL1) and VAL2 in Arabidopsis thaliana. We show that the val1 val2 double mutant possesses somatic embryonic phenotypes and a transcriptome strikingly similar to those of the swn clf double mutant, which lacks the PRC2 catalytic subunits SWINGER (SWN) and CURLY LEAF (CLF). We further show that VAL1 and V... More

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