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A TCR-like antibody against a proinsulin-containing fusion peptide ameliorates type 1 diabetes in NOD mice

Biochem Biophys Res Commun. 2020-11; 
Yushi Matsumoto, Kazuki Kishida, Maki Matsumoto, Sumiko Matsuoka, Masako Kohyama, Tadahiro Suenaga, Hisashi Arase
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Peptide Synthesis … pre-diabetic stage were weekly treated with 0.5 mg of the monoclonal antibody (3–5 … of latex beads (Thermo Fisher Scientific, A37304) with 0.2 mg of streptavidin (GenScript, Z02043) … 293T cells pulsed with peptides (40 μg/ml) were incubated with primary antibodies followed by … Get A Quote

摘要

Type 1 diabetes (T1D) is an autoimmune disease caused by destruction of insulin-producing β cells. The response of autoreactive T cells to β cell antigens plays a central role in the development of T1D. Recently, fusion peptides composed by insulin C-peptide fragments and other proteins were reported as β cell target antigens for diabetogenic CD4 T cells in non-obese diabetic (NOD) mice. In this study, we generated a T cell-receptor (TCR)-like monoclonal antibody (mAb) against a fusion peptide bound to major histocompatibility complex (MHC) class II component to elucidate the function of the fusion peptides in T1D. In addition, we developed a novel NFAT-GFP TCR reporter system to evaluate the TCR-like mAb. T... More

关键词

Fusion peptide, Hybrid peptide, MHC class II molecule, NFAT-GFP reporter T cell, TCR-Like antibody, Type 1 diabetes