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Topology, Antiviral Functional Residues and Mechanism of IFITM1

Viruses. 2020; 
Fang Sun, Zhiqiang Xia, Yuewen Han, Minjun Gao, Luyao Wang, Yingliang Wu, Jean-Marc Sabatier, Lixia Miao, Zhijian Cao
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Catalog Antibody … Dengue virus type 1–4 antibody (MA1-27093) was purchased from Invitrogen (Shanghai, China). Abs against Flag (F1804) and Myc (A00863-100) were purchased from Sigma–Aldrich (Shanghai, China) and GenScript (Nanjing, China), respectively … Get A Quote

摘要

Interferon-inducible transmembrane proteins (IFITM1/2/3) have been reported to suppress the entry of a wide range of viruses. However, their antiviral functional residues and specific mechanisms are still unclear. Here, we firstly resolved the topology of IFITM1 on the plasma membrane where N-terminus points into the cytoplasm and C-terminus resides extracellularly. Further, KRRK basic residues of IFITM1 locating at 62-67 of the conserved intracellular loop (CIL) were found to play a key role in the restriction on the Zika virus (ZIKV) and dengue virus (DENV). Similarly, KRRK basic residues of IFITM2/3 also contributed to suppressing ZIKV replication. Finally, IFITM1 was revealed to be capable of restricting th... More

关键词

IFITM1, antiviral functional residues, mechanism, topology