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APC/CFZR-1 regulates centrosomal ZYG-1 to limit centrosome number

The Company of Biologists . 2021-06; 
Jeffrey C. Medley, Joseph R. DiPanni, Luke Schira, Blake M. Shaffou, Brandon M. Sebou and Mi Hye Song
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摘要

Aberrant centrosome numbers are associated with human cancers. The levels of centrosome regulators positively correlate with centrosome number. Thus, tight control of centrosome protein levels is critical. In Caenorhabditis elegans, the anaphasepromoting complex/cyclosome and its co-activator FZR-1 (APC/ CFZR-1),aubiquitinligase,negativelyregulatescentrosomeassembly through SAS-5 degradation. In this study, we report the C. elegans ZYG-1 (Plk4 in humans) as a potential substrate of APC/CFZR-1. Inhibiting APC/CFZR-1 or mutating a ZYG-1 destruction (D)-box leads to elevated ZYG-1 levels at centrosomes, restoring bipolar spindles andembryonicviabilitytozyg-1mutants,suggestingthatAPC/CFZR-1 influences centrosomal Z... More

关键词

APC/C, FZR-1, C. elegans, Centrosome, Proteolysis, ZYG-1