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Identification of Circular RNAs Related to Vascular Endothelial Proliferation, Migration, and Angiogenesis After Spinal Cord Injury Using Microarray Analysis in Female Mice

Front Neurol. 2021-09; 
Xin Ye, Yilei Chen, Jiasheng Wang, Jian Chen, Ying Yao, Lin-Lin Wang, Fengdong Zhao
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Proteins, Expression, Isolation and Analysis … All samples (20 μl) were separated on a SurePAGE TM (Cat.M00653, GenScript ® , USA) and transferred onto nitrocellulose membranes. Membranes were blocked in 5% skimmed milk powder dissolved in TBS-T for 1 h. Mouse anti-CD31 (Cat# 12242S, ABclonal, 1:1000), … Get A Quote

摘要

Traumatic spinal cord injury (SCI) can result in severe disability and causes a considerable socio-economic burden worldwide. Circular RNAs (circRNAs) are important regulators of gene expression and pathological processes, and may represent therapeutic targets for SCI. To further evaluate the role of circRNAs in SCI, we elucidated circRNA expression profiles related to vascular endothelial proliferation, migration, and angiogenesis during the early stages of secondary injury in a mouse model of SCI. Microarray analysis was performed to investigate the circRNA expression patterns in the spinal cord 3 days after SCI in female mice. Bioinformatic analyses, including GO enrichment analysis, KEGG pathway analysis, ... More

关键词

angiogenesis, circular RNAs, migration, proliferation, spinal cord injury